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AAT Bioquest

FAQ

Posted on April 1, 2020
Are there any calcium indicators that don't require probenecid (PBC)?
Calcium dyescalcium fluxcalcium indicatorfluorescent calcium indicatorno probenecid


Posted on December 10, 2019
Why is probenecid used to conduct calcium flux assays when using fluorescent calcium indicators in certain cell types?
Ca2+calciumcalbrytecell assayprobenecidcalbryte 520no probenecidcalcium mobilitycalcium indicatororganic anion transporter


Posted on June 13, 2019
When performing calcium flux assay in living cells with fluorescent calcium indicators, can you use a fluorescent plate reader that does not have a kinetic reading function or a built-in liquid handler?
calciumCa2+calcium indicatorcalcium fluxcalcium mobilitycell assayfluorescent plate readerendpointno washno probenecidGPCR


Posted on April 1, 2019
How long after addition of agonist can the wells be read by using the Cell Meter™ No Wash and Probenecid-Free Endpoint Calcium Assay Kit (Cat# 36312)?
calciumCa2+calcium indicatorcalcium fluxcalcium mobilitycell assayfluorescent plate readerendpointno washno probenecidGPCRprobenecidorganic anion transporterfluorescent calcium indicator